Journal article
T-cell responses against rhinovirus species A and C in asthmatic and healthy children
Immunity, Inflammation and Disease, Vol.6(1), pp.143-153
2018
Abstract
Background
Infections by rhinovirus (RV) species A and C are the most common causes of exacerbations of asthma and a major cause of exacerbations of other acute and chronic respiratory diseases. Infections by both species are prevalent in pre-school and school-aged children and, particularly for RV-C, can cause severe symptoms and a need for hospitalization. While associations between RV infection and asthma are well established, the adaptive immune-mechanisms by which RV infections influence asthma exacerbations are yet to be defined.
Objective
The aim of this study was to characterize and compare T-cell responses between RV-A and RV-C and to test the hypothesis that T-cell responses would differ between asthmatic children and healthy controls.
Methods
A multi-parameter flow cytometry assay was used to characterize the in vitro recall T-cell response against RV-A and RV-C in PBMCs from children with acute asthma (n = 22) and controls (n = 26). The responses were induced by pools of peptides containing species-specific VP1 epitopes of RV-A and RV-C.
Results
Regardless of children's clinical status, all children that responded to the in vitro stimulation (>90%) had a similar magnitude of CD4+ T-cell responses to RV-A and RV-C. However, asthmatic children had a significantly lower number of circulating regulatory T cells (Tregs), and healthy controls had significantly more Tregs induced by RV-A than RV-C.
Conclusions and Clinical Relevance
The comparable recall memory T-cell responses in asthmatic and control children to both RV-A and RV-C show that differences in the antibody and inflammatory responses previously described are likely to be due to regulation, with a demonstrated candidate being reduced regulatory T-cells. The reduced Treg numbers demonstrated here could explain the asthmatic's inability to appropriately control immunopathological responses to RV infections.
Details
- Title
- T-cell responses against rhinovirus species A and C in asthmatic and healthy children
- Authors/Creators
- C.M. Gaido (Author/Creator) - The Kids Research Institute AustraliaC. Granland (Author/Creator) - The Kids Research Institute AustraliaI.A. Laing (Author/Creator) - The Kids Research Institute AustraliaP.N. Le Souëf (Author/Creator) - Princess Margaret Hospital for ChildrenW.R. Thomas (Author/Creator) - The Kids Research Institute AustraliaA.J. Currie (Author/Creator) - School of Paediatrics and Child HealthThe University of Western AustraliaPerthAustraliaB.J. Hales (Author/Creator) - The Kids Research Institute Australia
- Publication Details
- Immunity, Inflammation and Disease, Vol.6(1), pp.143-153
- Publisher
- John Wiley & Sons Ltd
- Identifiers
- 991005542027907891
- Copyright
- © 2017 The Authors
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.104 Virology - General
- 1.104.975 Respiratory Viral Infections
- Web Of Science research areas
- Immunology
- ESI research areas
- Immunology