Michael Dilworth

Summary This manual brings together state-of-the-art methods for the study of root-nodule bacteria, both in the free-living state and in symbiosis with legumes. In each chapter, the manual introduces a topic and provides guidance on how study of the symbiosis might best be tackled. Detailed descriptions of the protocols that need to be followed, potential problems and pitfalls are provided. Topics covered include acquiring, recognising, growing and storing rhizobia, experimenting with strains in the laboratory, glasshouse and field, and applying contemporary molecular and genetic methodologies to assist in the study of rhizobia.