Output list
Book chapter
Towards developing transgenic resistance to nematodes in wheat
Published 2009
Cereal cyst nematodes: Status, research and outlook. Proceedings of the first Workshop of the International Cereal Cyst Nematode Initiative, 21-23 October 2009, Antalya, Turkey, 191 - 194
Research is in progress to use biotechnological tools to aid development of new forms of diagnostics and resistance to nematodes in wheat. For diagnostics, protein profiling by MALDI-TOF mass spectroscopy has been used to identify a range of nematode species, including seed gall, root-lesion and stem nematodes. Nematode species, and in some cases, races, can be identified and differentiated on the basis of characteristic protein biomarkers. A reproducible assay system for compounds that affect root-lesion nematode replication, based on carrot mini-discs, has been developed. Genomic studies have also been initiated to generate eDNA libraries and genomic sequences of root lesion and cereal cyst-nematodes. For new forms of genetic resistance to nematode pests of cereals, molecular approaches are in progress to identify new gene targets, generation of potential resistance-conferring gene constructs and their introduction into wheat by particle bombardment followed by regeneration and challenge to identify nematode resistant wheat plants.
Book chapter
Published 1999
Across Century Biological and Medicine Industry Developing Research and Environmental Protection, 215 - 218
Book chapter
Production of virus-free economic crop in factory
Published 1999
Across Century Biological and Medicine Industry Developing Research and Environmental Protection, 177 - 180