Tona Sanchez-Palacios

Meteoric-10Be has become a popular proxy for assessing glacial environments and processes around Antarctica, such as meltwater discharge or ice shelf environments. Despite applications in recent paleostudies, little testing of the mechanisms driving the deposition of Be-isotopes into marine sediments has been conducted. We used chemical leach procedures to sequentially or partially extract 10Be and 9Be from bulk sediments to assess the possible sources and depositional processes affecting them. Additionally, we leached the reactive phase of five different grainsize splits to determine whether 10Be/9Be ratios normalise for grainsize effects acting upon the 10Be concentration. Reactive Be-isotopes are primarily situated in the oxide phases of sediments, with the amorphous oxide (Am-Ox) phases consisting of much higher 10Be/9Be ratios (~7–10 × 10−8) than the crystalline oxides (~1–3 × 10−8; X-Ox), indicating that the Am-Ox phase better represents authigenic oxide production and a circumpolar deep water source, which is contrary to most of the current literature. Published leach procedures targeting the reactive phase of sediment consist of ratios in between the Am-Ox and X-Ox phases (~3–7 × 10−8), indicating that they target both phases to some degree. The fractionation of Be-isotopes in Antarctic sediment samples shows that circumpolar deep water is the primary source of 10Be, and that the “reactive” signatures from different leach steps targeting the reactive phase are not the same.

Globally, zinc (Zn) deficiency in soils, and subsequently crops, has emerged as one of the most prevalent among micronutrients, resulting in a severe decline in crop yields and nutritional quality and in adversely affecting animal and human health. Worldwide, more than half of the agricultural soils are inherently deficient in Zn, and the health of about one-third of the global human population is impacted by Zn deficiency. Zinc is an essential micronutrient for animal and human health, and, in the developing world, Zn deficiency has been identified as the fifth cause of disease and death for humans. The World Health Organization (WHO) reports that annually more than 800,000 people, including around 450,000 children under the age of 5, die due to Zn deficiency. Zinc supplementation was frequently associated with boosting immunity against COVID-19 in recent years. Because most of the Zn in animals and humans is derived from soil-grown crops, their source of Zn is highly dependent on plant Zn, especially in crops or fodder; in turn, crop Zn is dependent on available soil Zn levels. This integrated review describes Zn distribution, behavior, and fate in soils and its uptake and role in plants and crop production, as well as in the well-being of animals and humans. It discusses recent findings concerning Zn deficiency in all steps of the human food chain (from soil, to crop, animal, and human), and how it can be addressed through novel Zn fertilizers, soil amendments, and biofortification of Zn.

Iodine is an essential micronutrient for human nutrition, though it is found in relatively low concentrations in many important crop species. Wheat (Triticum aestivum L.) is a common staple crop worldwide, and as such could be an important source of dietary iodine due to its widespread consumption. However, little is known about iodine concentrations in wheat grain grown under rainfed field conditions, nor the impact of growing region or environment on these concentrations. Therefore, this paper had three objectives; (1) quantify the iodine concentration in a popular variety of wheat cultivated across the wheat belt of three Australian States (Western Australia, South Australia and Victoria) over two winter seasons (2) determine the influence of distance from the coast, rainfall, elevation, soil type and pH and grain yield on wheat grain iodine concentrations and (3) identify geographical areas where iodine concentrations of wheat grains are low enough that biofortification with iodine would be advantageous for human health outcomes. We sampled iodine concentrations of a single cv. Scepter at 125 sites from the winter season 2020 (65 sites) and 2021(60 sites), to investigate environmental and geographical effects on wheat grain iodine concentrations. Iodine concentrations were measured using triple quadrupole inductively coupled plasma mass spectrometer (ICP MS/MS). We found that the elevation and the region (State) of growing sites were the most significant predictors of iodine concentration, along with the interaction between rainfall and topsoil texture. However, very low concentrations of iodine (5–24 μg/kg) were detected in all samples tested, indicating that even wheat grown under advantageous environmental and geographic conditions in southern Australia would be unlikely to represent an important source of dietary iodine. This emphasises the need to consider biofortification strategies to improve iodine concentrations in Australian grown wheat to improve the dietary uptake of this essential micronutrient by human consumers.

Beryllium-10 (10Be) has been proposed as a potential proxy for investigating ice shelf presence and absence, or meltwater discharge in coastal polar environments. However, the sources and distribution of atmospherically produced meteoric-10Be in the Antarctic marine realm are yet to be fully characterized, making any inferences about its concentration in sediments challenging. We present a dataset of 9Be and 10Be concentrations, and 10Be/9Be ratios in seafloor surface sediments from the Antarctic continental shelf - including from sub ice shelf cores - to assess the sources and processes contributing Be-isotopes to ice-sheet proximal marine settings. We show that upwelling waters (e.g. Circumpolar Deep Water) are a significant source of 10Be to continental shelf sediments. This limits the use of 10Be/9Be as a proxy for ice shelf environment or meltwater discharge, but instead provides a potential proxy for reconstructing Circumpolar Deep Water incursions onto Antarctic continental shelves.

Over eight million hectares of land in south-west Western Australia required applications of micronutrients, particularly copper, zinc and molybdenum, in order to grow profitable crops and pastures when first cleared for agricultural production. Initial fertiliser applications, particularly of copper and zinc, had very long residual effectiveness – research showed that the once-off application of copper continued to correct crop deficiencies for over 30 years.

Agronomic biofortification of crops is a promising approach that can improve the nutritional value of staple foods by alleviating dietary micronutrient deficiencies. Iodine deficiency is prevalent in many countries, including Australia, but it is not clear what foliar application strategies will be effective for iodine fortification of grain. This study hypothesised that combining adjuvants with iodine in foliar sprays would improve iodine penetration in wheat, leading to more efficient biofortification of grains. The glasshouse experiment included a total of nine treatments, including three reference controls: 1) Water; 2) potassium iodate (KIO3) and 3) potassium chloride (KCl); and a series of six different non-ionic surfactant or oil-based adjuvants: 4) KIO3 + BS1000; 5) KIO3 + Pulse® Penetrant; 6) KIO3 + Uptake®; 7) KIO3 + Hot-Up®; 8) KIO3 + Hasten® and 9) KIO3 + Synerterol® Horti Oil. Wheat was treated at heading, and again during the early milk growth stage. Adding the organosilicon-based adjuvant (Pulse®) to the spray formulation resulted in a significant increase in grain loading of iodine to 1269 µg/kg compared to the non-adjuvant KIO3 control at 231µg/kg, and the water and KCl controls (both 51µg/kg). The second most effective adjuvant was Synerterol® Horti Oil, which increased grain iodine significantly to 450µg/kg. The Uptake®, BS1000, Hasten®, and Hot-Up® adjuvants did not affect grain iodine concentrations relative to the KIO3 control. Importantly, iodine application and the subsequent increase in grain iodine had no significant effects on biomass production and grain yield relative to the controls. These results indicate that adjuvants can play an important role in agronomic biofortification practices, and organosilicon-based products have a great potential to enhance foliar penetration resulting in a higher translocation rate of foliar-applied iodine to grains, which is required to increase the iodine density of staple grains effectively.

Agronomic biofortification of wheat grain with zinc can improve the condition of about one billion people suffering from zinc (Zn) deficiency. However, with the challenge of cultivating high-yielding wheat varieties in Zn-deficient soils and the global need to produce higher-quality food that nourishes the growing population, innovation in the strategies to deliver Zn directly to plants will come into play. Consequently, existing foliar formulations will need further refinement to maintain the high agronomic productivity required in competitive global grain markets while meeting the dietary Zn intake levels recommended for humans. A new generation of foliar fertilisers that increase the amount of Zn assimilated in wheat plants and the translocation efficiency of Zn from leaves to grains can be a promising solution. Research on the efficacy of adjuvants and emerging nano-transporters relative to conventional Zn forms applied as foliar fertilisers to wheat has expanded rapidly in recent years. This review scopes the range of evidence available in the literature regarding the biofortification of bread wheat (Triticum aestivum L.) resulting from foliar applications of conventional Zn forms, Zn nanoparticles and novel Zn-foliar formulations. We examine the foliar application strategies and the attained final concentration of grain Zn. We propose a conceptual model for the response of grain Zn biofortification of wheat to foliar Zn application rates. This review discusses some physiological aspects of transportation of foliarly applied Zn that need further investigation. Finally, we explore the prospects of engineering foliar nano-formulations that could effectively overcome the physicochemical barrier to delivering Zn to wheat grains.

Arsenic in marine environment is ubiquitous, yet it can be toxic to living organisms. Arsenic enters the marine food chain through algae. Algae convert the accumulated inorganic containing As molecules into methylated forms and more complex molecules such as carbohydrates (i.e. arsenoribosides), fatty acids and other organic species. Arsenoribosides can make up 70% of the total accumulated As in algae. When transferred to herbivorous fish such as members of the family Kyphosidae, arsenoribosides can be converted into less toxic forms such as thio-, glycerol-, phosphate- and methylated-arsenoribosides or substantially converted into arsenobetaine. Much remains to be understood however regarding the transfer and metabolism of arsenoribosides in marine organisms. In this study, we proposed to conduct As-speciation analyses in the intestine system of Kyphosus spp., exposed to As-containing seaweed. Extraction and separation analytical techniques for As-speciation such as HPLC-HG-AFS, HPLC-ICPMS and LC-MS-MS will be employed for As containing metabolites determination. It is hypothesised that changes in proportion of As metabolites will be observed along the intestine of Kyphosus spp.

Bread wheat (Triticum aestivum L.) is cultivated on more land than any other crop and produces a fifth of the calories consumed by humans. Wheat endosperm is rich in starch yet contains low concentrations of dietary iron (Fe) and zinc (Zn). Biofortification is a micronutrient intervention aimed at increasing the density and bioavailability of essential vitamins and minerals in staple crops; Fe biofortification of wheat has proved challenging. In this study we employed constitutive expression (CE) of the rice (Oryza sativa L.) nicotianamine synthase 2 (OsNAS2) gene in bread wheat to up-regulate biosynthesis of two low molecular weight metal chelators - nicotianamine (NA) and 2'-deoxymugineic acid (DMA) - that play key roles in metal transport and nutrition. The CE-OsNAS2 plants accumulated higher concentrations of grain Fe, Zn, NA and DMA and synchrotron X-ray fluorescence microscopy (XFM) revealed enhanced localization of Fe and Zn in endosperm and crease tissues, respectively. Iron bioavailability was increased in white flour milled from field-grown CE-OsNAS2 grain and positively correlated with NA and DMA concentrations.